Dr. Manoj K. Mishra
Manoj K. Mishra, Ph.D.
Professor of Biology
Director, Cancer Biology Research and Training Program
Director, Freshman Biology Program
Department of Biological Sciences
Dr. Mishra received his M.Sc. and Ph.D. degrees from Banaras Hindu University, Varanasi, India. After obtaining his Ph. D., he moved to United States and did his postdoctoral training in the Division of Biology, Kansas State University, Manhattan, KS; Division of Allergy and Immunology, Department of Pediatrics, Washington University School of Medicine, St. Louis, MO; and Division of Rheumatology, Department of Pediatrics at Medical College of Wisconsin, Milwaukee, WI. In the year 2006, he continued at Medical College of Wisconsin and moved in the Division of Allergy and Clinical Immunology within the Department of Pediatrics as a Research Scientist before coming to Alabama State University in February 2009, where he holds an Associate Professor of Biology position in the Department of Biological Sciences. Additionally, Dr. Mishra also serves as the Director for Cancer Biology Research and Training Program and Freshman Biology Program at ASU. Since February 2011, Dr. Mishra also holds an adjunct Professor Position in the Division of Molecular and Cellular Pathology within the Department of Pathology, School of Medicine at the University of Alabama at Birmingham, AL. Dr. Mishra lab is currently supported by research and educational grants from US Department of Defense and National Science Foundation. In 2013, Dr. Mishra has recognized as Top 25 professors at HBCU in the USA and also received awards for his research work at ASU. In 2015, Dr. Mishra received STEM faculty of the year award at Alabama State University.
Project 1- Role of dietary compounds in controlling prostate cancer development and progression: Our current research interest is to understand the molecular mechanism(s) involved in prostate cancer development and clearance in response to a dietary compound, resveratrol (RES). Resveratrol is a polyphenolic compound found in the skin of red fruits, exhibits anti-inflammatory, anti-oxidative, anti-cancer, and anti-proliferative characteristics. RES was first detected in the dried roots of Polygonum cuspidatum, traditionally used in Chinese and Japanese medicines as an anti-inflammatory agent. This project focuses on to explore the role RES on NK cells, macrophages, particularly the tumor-associated macrophages (TAM), and regulatory T cells in tumor microenvironment.
Project 2- Role of immune cells in prostate cancer: Prostate Cancer (PCa) is the most common non-skin malignancy and commonly diagnosed cancer, and second most cancer responsible for death in men in the United States. Prior studies in animal models of PCa as well as in human, demonstrated that the prostate produces several growth factors. Addition or blocking of these growth factors can alter the PCa cell proliferation and other important functions. Among these growth factors, transforming growth factor-β1 (TGF-β) plays a significant role in tumorigenicity, displays potent immunosuppressive activities, and is required for the conversion of conventional CD4+ T cells to FoxP3+ regulatory T cells (TR). TR that expresses the transcription factor Foxp3 is essential for normal immune function. The absence of TR cells results in multi-organ autoimmunity and death. A clear role of TGF-β and its effect on TR during PCa lacks experimental evidence. In this project, we are investigating the underlying mechanisms involved in the context of TGF-β and/or TR using PCa cell lines, derived from the transgenic mouse for prostate cancer (TRAMP), in C57/B6 mice. These cell lines, TRAMP-C1 and TRAMP-C2 form tumors while TRAMP-C3 fails to form tumors. The objective of this project is to define the role of TGF-β production by host cells in response to or TGF-β expression by TRAMP cell lines and to analyze the role of TGF-β in the conversion of naïve CD4+ T cells to Foxp3 expressing TR cells. This project is currently funded by a P20 grant from NCI, NIH. For more information about this project click here.
Project 3- Role of polymeric scaffolds in bone tissue regeneration: The natural ECM plays an important role in morphogenesis, tissue development, and angiogenesis since it acts as binding sites for many proteins such as cytokine molecules and growth factors. This project is designed to study the role of multifunctional polymeric scaffolds that mimic the characteristics of natural extracellular matrix (ECM). The goal of this project is to further mimic the natural ECM by understanding the fundamental mechanisms involved in the adsorption and release of therapeutics from polymeric tissue scaffolds. This understanding will aid in the development of new drug-eluting tissue scaffolds. This project is currently funded by a research grant from US Department of Defense. For more information about this project click here
Recent Publications (2009-onwards)
1. Abron JD, Singh NP, Murphy AE, Mishra MK, Price RL, Nagarkatti M, Nagarkatti PS, Singh UP (2018) Differential role of CXCR3 in inflammation and colorectal cancer. Oncotarget. 9(25):17928-17936 PMID: 29707158.
2. Abron JD, Singh NP, Mishra MK, Price RL, Nagarkatti M, Nagarkatti PS, Singh UP (2018) An endogenous aryl hydrocarbon receptor (AhR) ligand, ITE induces regulatory T cells (Tregs) and ameliorates experimental colitis. Am J Physiol Gastrointest Liver Physiol. doi: 10.1152/ajpgi.00413.2017 PMID: 29672155.
3. Andrew Wood, Dominique Everett, Sanjay Kumar, Manoj Mishra, and Vinoy Thomas (2018) Fiber Length and Concentration: Synergistic Effect on Mechanical and Cellular Response in Wet-Laid poly(lactic acid) Fibrous Scaffolds. J Biomed Mater Res B Appl Biomater. doi: 10.1002/jbm.b.34125 PMID: 29656479.
4. Sanjay Kumar, Sanjeev Gurshaney, Yori Adagunodo, Erica Gage, Shezreen Qadri, Mahak Sharma, Shalie Malik, Upender Manne, Udai P. Singh, Rajesh Singh, Manoj K. Mishra (2018). Hsp70 and γ-Semino Protein as possible prognostic marker of prostate cancer. Frontiers in Biosciences. 987-2000.
5. Singh SK, Mishra MK, Eltoum IA, Bae S, Lillard JW Jr., Singh R. (2018) CCR5/CCL5 axis interaction promotes migratory and invasiveness of pancreatic cancer cells. Sci Rep. 2018 Jan 22;8(1):1323. PMCID: PMC5778036
6. Sanjay Kumar, Rajesh Singh, Shalie Malik, Upender Manne, Manoj Mishra (2017) Prostate cancer health disparities: An immuno-biological perspective. Cancer Letters. 414:153-165, PMID: 29154974.
7. Bo Wang, Wuwei Li, Derrick Dean, Manoj K. Mishra and Kennedy S. Wekesa (2017) Enhanced Hepatogenic Differentiation of Bone Marrow Derived Mesenchymal Stem Cells on Liver ECM Hydrogel. Journal of Biomedical Materials Research Part A. Published online on October 25, 2017, DOI: 10.1002/jbm.a.36278
8. Sanjay Kumar, James Stokes III, Udai P Singh, Karyn Scissum-Gunn, Rajesh Singh, Upender Manne, Manoj K Mishra (2017) Prolonged exposure of resveratrol induces reactive superoxide species–independent apoptosis in murine prostate cells. Tumor Biology. 39(10): 1-10. DOI: 10.1177/1010428317715039 [PMCID not found]
9. Kumar S, Eroglu E, Stokes III JA, Gunn KS, Saldanha SN, Singh UP, Manne U, Ponnazhagan S, Mishra MK. (2017) Resveratrol induces mitochondria-mediated, caspase-independent apoptosis in murine prostate cancer cells. Oncotarget. 8(13):20895-20908, PMCID: PMC5400554
10. Shamran H, Singh NP, Zumbrun EE, Murphy A, Taub DD, Mishra MK, Price RL, Chatterjee S, Nagarkatti M, Nagarkatti PS, Singh UP (2017) Fatty acid amide hydrolase (FAAH) blockade ameliorates experimental colitis by altering microRNA expression and suppressing inflammation. Brain, Behavior and Immunity. 59:10-20, PMCID: PMC5154806
11. Sanjay Kumar, James A. Stokes III, Derrick Dean, Christian Rogers, Elijah Nyario, Vinoy Thomas, and Manoj K. Mishra (2017) Biphasic organo-bioceramic fibrous composite as a biomimetic extracellular matrix for bone tissue regeneration. Frontiers in Biosciences. 9: 192-200. PMID: 28199184
12. Anil Shanker, Menaka Thounaojam, Manoj K Mishra, Mikhail M Dikov (2017) Innate-Adaptive Immune Crosstalk 2016. Journal of Immunology Research. 1-2. PMCID: PMC5405582
13. Sanjay Kumar, James Stokes III, Udai P. Singh, Karyn Scissum Gunn, Arbind Acharya, Upender Manne, Manoj Mishra (2016) Targeting Hsp70: A possible therapy for cancer. Cancer letters. 374:156–166. PMID: 26898980
14. Anil Shanker, Menaka C Thounaojam, Manoj K. Mishra, Mikhail M. Dikov and Roman V. Uzhachenko (2015) Innate-Adaptive Immune Crosstalk. Journal of Immunology Research. 1-2. PMCID: PMC5405582
15. Maria Teresa P. de Aquino, Anshu Malhotra, Manoj K. Mishra and Anil Shanker (2015) Challenges and future perspectives of T cell immunotherapy in cancer. Immunology Letters. 19; 166(2):117-133. PMCID:PMC4499494
16. Udai P. Singh, Angela E. Murphy, Reilly T. Enos, Haidar A. Shamran, Narendra P. Singh, Honbing Guan, Venkatesh L. Hegde, Daping Fan, Robert L. Price, Dennis D. Taub, Manoj K. Mishra, Mitzi Nagarkatti, and Prakash S. Nagarkatti (2014) MicroRNA-155 deficiency protects mice from experimental colitis by reducing Th1/Th17 responses. Immunology. 143(3):478-489. PMCID:PMC4212960
17. Amanee D. Salaam, Manoj K. Mishra, Elijah Nyairo and Derrick R. Dean. (2014) Electrospun polyvinyl alcohol/nanodiamond composite scaffolds: morphological, structural, and biological analysis. Journal ofBiomaterials and Tissue Engineering. 4:73-180. DOI: 10.1166/jbt.2014.1152
18. Singh UP, Singh NP, Guan H, Busbee B, Price RL, Taub DD, Mishra MK, Fayad R, Nagarkatti M, Nagarkatti PS. (2014) The Emerging Role of Leptin Antagonist as Potential Therapeutic Option for Inflammatory Bowel Disease. Int Rev Immunol. 33:(1):23-33. PMCID:PMC4159716
19. James Stokes III, Udai P. Singh, Elijah Nyairo, Manoj K. Mishra (2013) Resveratrol and its impact on Prostate cancer. International Journal of Advanced Biotechnology & Bioinformatics. 2:6-13. [not found]
20. Erica G. Schmitt, Dipica Haribhai, Jonathan C. Jeschke, Dominic O. Co, Jennifer Ziegelbauer, Ke Yan, Yoichiro Iwakura, Manoj K. Mishra, Pippa Simpson, Nita H. Salzman, and Calvin B. Williams (2013) Chronic Follicular Bronchiolitis Requires Antigen-Specific Regulatory T Cell Control to Prevent Fatal Disease Progression. Journal of Immunology. 191:5460-5476. PMCID: PMC3893829
21. Udai P. Singh, Narendra P. Singh, Honbing Guan, Venkatesh L. Hegde, Robert L. Price, Dennis D Taub, Manoj K. Mishra, Mitzi Nagarkatti, & Prakash S. Nagarkatti (2013) The severity of experimental autoimmune cystitis can be ameliorated by anti-CXCL10 Ab treatment. PLOS One, 8(11):e79751. PMCID: PMC3836899
22. Singh UP, Singh NP, Guan H, Busbee B, Price RL, Taub DD, Mishra MK, Fayad R, Nagarkatti M, Nagarkatti PS. (2013) Leptin antagonist ameliorates chronic colitis in IL-10-/- mice. Immunobiology. 218(12):1439-1451. PMCID: PMC3778116
23. Udai P. Singh, Narendra P. Singh, Brandon Busbee, Guan, H, Robert L. Price, Dennis D. Taub, Manoj K. Mishra, Mitzi Nagarkatti, and Prakash S. Nagarkatti (2012) Microbial Links to Inflammatory Bowel Disease Development: Potential Interventional Strategies in Treatment. Journal of Bacteriology and parasitology. 3:7. [not found]
24. Renu Bhatt, Vinoy Thomas, Derrick Dean, Elijah Nyairo, Udai P. Singh, and Manoj K. Mishra (2012) Role of PPARs as Chemotherapeutics and Anticancer molecules. International Journal of Advanced Biotechnology & Bioinformatics. 1:11-18. [not found]
25. Himani D. Deshpande, Derrick R. Dean, Vinoy Thomas, William C. Clem, Moncy V. Jose, Elijah Nyairo, and Manoj K. Mishra. (2012) Carbon nanofiber reinforced polycaprolactone fibrous meshes by electrostatic co-spinning. Current Nanoscience. 8(6):753-761. DOI: 10.2174/157341312802884409
26. Amanee Salaam, Manoj K. Mishra, Elijah Nyairo, and Derrick Dean (2012) Morphological and Biological Characterization of PVA-Nanodiamond Electrospun Fibers. Polymeric Materials: Science and Engineering. 106:334-335. doi:10.1166/jbt.2014.1152
27. Udai P. Singh, Narendra P. Singh, Brandon Busbee, Guan, H, Balwan Singh, Robert L. Price, Dennis D. Taub, Manoj K. Mishra, Mitzi Nagarkatti, and Prakash S. Nagarkatti (2012) Alternative medicines as emerging therapies for inflammatory bowel diseases. International Reviews of Immunology. 31(1):66-84. PMCID: PMC4138959
28. Himani Deshpande, Carrie Schindler, Derrick Dean, William Clem, Susan Bellis, Elijah Nyairo, Manoj K. Mishra, Vinoy Thomas (2011) Nanocomposite scaffolds based on electrospun polycaprolactone/modified carbon nanofiber/ nanohydroxyapatite by electrosphoretic deposition. Journal of Biomaterials and Tissue Engineering. 1:177-184. DOI: 10.1166/jbt.2011.1024
29. Udai P. Singh, Narendra P. Singh, Balwan Singh, Manoj K. Mishra, Mitzi Nagarkatti, Prakash S. Nagarkatti, and Singh S.R. (2010) Stem Cell in Inflammatory Bowel Disease and its Therapeutic Implication. Frontiers in Bioscience. 1(2):993-1008. [not found]
30. Srikanta Jana, Parthav Jailwala, Dipica Haribhai, Jill Waukau, S Glisic, William Grossman, Manoj K. Mishra, Ren Wen, D Wang, Calvin B Williams, S Ghosh. (2009) The role of NF-kappaB and Smad3 in TGF-beta-mediated Foxp3 expression. European Journal of Immunology. 39(9):2571-83. PMID:19701891
31. Lance M. Relland, Manoj K. Mishra, Dipica Haribhai, Brandon Edwards, Jennifer Zeigelbauer, and Calvin B. Williams (2009). Affinity-based selection of regulatory T cells occur independent of agonist-mediated induction of Foxp3 expression. Journal of Immunology. 182(3):1341-1350. PMCID: PMC2818884
1. Sanjay Kumar, Rajesh Singh, Manoj K. Mishra (2017). Therapeutic routes for pancreatic cancer management. Elsevier publication, Saldanha S. Ed. [in press].
2. Shriti Singh, Manoj Kumar Mishra and Rajesh Singh (2017). Epigenetic Modulations in Ovarian Cancer. Elsevier publication, Saldanha S. Ed. [in press].
3. Sanjay Kumar, James A. Stokes III, Udai P. Singh, and Manoj K. Mishra (2016) Ezh2, an epigenetic regulator: The future of prostate cancer treatment. In: Epigenetic Advancements in Cancer. Editors-Mishra and Bishnupuri, Springer International Publishing AG, Gewerbestrasse 11, 6330 Cham, Switzerland, 2016; p229-245.
4. James A. Stokes III, Sanjay Kumar, Karyn Scissum-Gunn, Udai P. Singh, and Manoj K. Mishra (2016) Epigenetic and cancer: An evaluation of the impact of dietary components. In: Epigenetic Advancements in Cancer. Editors-Mishra and Bishnupuri, Springer International Publishing AG, Gewerbestrasse 11, 6330 Cham, Switzerland, 2016; p65-78.
5. Kumar S Bishnupuri and Manoj K. Mishra (2016) Epigenetics of Colorectal Cancer. In: Epigenetic Advancements in Cancer. Editors-Mishra and Bishnupuri, Springer International Publishing AG, Gewerbestrasse 11, 6330 Cham, Switzerland.
6. James A. Stokes III and Manoj K. Mishra (2014) Role of resveratrol (RES) in regenerative medicine. In: Diverse Applications of Nanotechnology in Biomedicine, Chemistry, and Engineering. IGI Global, Hershey, PA Chapter 18, pages 392-402.
7. Shree Ram Singh, Manoj K. Mishra and Chhavi Chauhan (2012) Cell Transplantation in Chronic Kidney Disease. In: Cell Transplantation: New Research, Nova Science Publishers, Inc., New York, 978-1-62100-542-1, Page 1-24.
8. Shree Ram Singh, Manoj K. Mishra, Maduri Kango-Singh and Steven X. Hou (2012) Generation and Staining of Intestinal Stem Cell Lineage in Adult Midgut. In: Methods in Molecular Series, Somatic STEM cells: Methods in Molecular Biology, 879: 47-69.
9. Shree Ram Singh and Manoj K. Mishra (2012) JAK/STAT Signaling in Cell Fate and Tumorigenesis. In: Signaling, gene regulation and cancer, Nova Science Publishers, Inc., New York, ISBN: 978-1-61942-092-2, Chapter 4, pages 63-72.